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MagicBlueTM Rapid Protein Stain Solution

- Product Highlight
- Unique Features
- Product Pictures
- Ordering Information

Product Highlight
MagicBlueTM is a proprietary formulation of coomassie blue G-250 solution.  It is based on a new heat-enhanced, colloidal coomassie blue staining technology.   Its unique formulation allows detection of proteins in native and SDS-PAGE gels in 10 minutes using a simple procedure.  No destaining step is needed after the staining step.  MagicBlueTM formulation is environment-friendly and does not contain hazardous acid such as acetic, hydrochloric, phosphoric and perchloric acid, and flammable chemicals such as methanol and ethanol.


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Unique Features:
-   One step procedure without destaining requirement for protein visualization
-   Takes 10 minutes to stain a protein gel.
-   
Detects as little as 10 ng* of protein in a gel.
-  
Linear detection between 50 ng and 4 mg of protein.
-   Based on a new heat-enhanced, colloidal coomassie blue staining technology.
-   Environmentally-safe formula.
-   No flammable and hazardous chemicals such as ethanol, methanol, acetic acid,
    hydrochloric acid, perchloric acid and phosphoric acid.

* Based on BSA ran on a 12-wells mini polyacrylamide gel.  Detection sensitivity varies from protein to protein.  Best for proteins 15 k Da and above.

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Product Pictures :

A.

B.

    I.                                                                                               II.

        

Figures: Detection of proteins by MagicBlueTM 4 - 20% Tris-Glycine mini gels were stained with MagicBlueTM. Figure BI. Lanes 1 and 2 represent 100 and 200 ng of BSA. Lanes 3 - 4 represent 100 and 200 ng of glutathione S-transferase. Lanes 5 and 6 represent 2 and 4 mg of marker proteins of 200, 97, 68, 43, 30, 18 and 14 k Da. Lanes 7 - 9 represent 2.5, 5 and 10 mg of total bacterial lysate. Lanes 10 - 12 represent 2, 4 and 8 mg of total cell lysate from NIH 3T3 cells. Figure BII. Lanes 1 - 8 represents 2 mg, 1 mg, 500, 250, 100, 50, 25 and 12.5 ng of BSA.

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Ordering Information
MB100         MagicBlueTM rapid protein stain solution                        1000 ml
MB102         MagicBlueTM Starter Kit
                      (Kit contains 1 liter of MagicBlueTM rapid protein
                      stain solution and 1 MagicBlueTM stain tray)


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Frequently Asked Questions (pdf)

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Product Information (pdf)

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