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GoldRunnerTM Rapid Electrophoresis Buffer for Agarose Gel Analysis
GoldRunnerTM rapid electrophoresis buffer is a new generation of agarose gel electrophoresis buffer with an unique formulation for rapid gel analysis.   It allows gel electrophoresis to be performed in high voltage without the problems (melting of the gel, DNA bands smiling, decrease of DNA bands resolution) caused by the high heat and high current associated with the traditional TAE and TBE buffer.  An agarose gel electrophoresis performed in GoldRunnerTM rapid electrophoresis buffer can be completed in 1/2 to 1/3 of the time required for a gel performed in the traditional TAE or TBE buffer. 

                1% TAE agarose gel        1% GoldRunnerTM agarose gel

                     60 min               90 min                            30 min

                          

Figure:  1 kb DNA marker and 100 bp DNA marker were run on a 1% mini agarose gel prepared in TAE buffer (two gels on left), or GoldRunnerTM rapid electrophoresis buffer (right).  The TAE gel was performed at constant 80 volts and the GoldRunnerTM gel was performed at constant 250 volts for the time indicated.  The bands in the 1 kb marker lane are 10.2, 8, 6, 5, 4, 3, 2, 1.6, 1 and 0.5 kb.  The bands in the 100 bp marker lane are 2000, 1600, 1200, 1000, 900, 800, 700, 600, 500, 400, 300, 200 and 100 bp.

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New Services
Express Plasmid Purification Service:
This low cost, fast turnaround, 250 ml-scale (equals to maxi prep) purification service is tailored for researcher who needs small amount of DNA for their resaerch. The service uses column-based purification method for plasmid less than 20 kb. Plasmid DNA purified by express quality service typically contains 5 - 10 EU/ug of DNA and is suitable for many applications such as probe synthesis, in vitro RNA synthesis, DNA cloning, DNA sequencing, bacterial and yeast transformation.
Restriction analysis is not included in this service. Production scale: 250 ml.

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Express Plus Plasmid Purification Service:
This low cost, fast turnaround, 250 ml-scale (equals to maxi prep) purification service is tailored for researcher who needs small amount of DNA for their resaerch. The service uses Qiagen’s Endo-Free column-based purification method for plasmid less than 20 kb. Plasmid DNA purified by Express Plus quality service typically contains 0.1 EU/ug of DNA and is recommended for endotoxin-critical applications such as transfection of mammalian cells and animal-related experiments. Restriction analysis is not included in this service.Production scale: 250 ml.

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